application note:
High Speed Orbital Shaking
The growth of low-volume bacterial cultures is advantageous for reducing material costs and increasing analytical throughput. Standard 96-well plates are often used for bacterial growth experiments, but achieving sufficient oxygen transfer rates for aerobic growth requires rapid sample agitation. Utilizing the Stratus Plate Reader (Cerillo Inc., USA) with the MS 3 Basic (IKA, Germany), this application note details the result of rapidly shaking E. coli K12 cultures within the Stratus Plate Reader to achieve continuously monitored aerobic bacterial growth curves.
In this application note you will learn:
- The effect rapid orbital shaking has on E. coli growth cultures
- How to determine orbital shaking efficacy
